Fisheries Fisheries Рыбное хозяйство 0131-6184 52347 10.37663/0131-6184-2022-4-71-75 Аквакультура Aquaculture Аквакультура Evaluation of the genotoxicity of diflubenzuron by micronucleus test on red blood cells Danio rerio Оценка генотоксичности дифлубензурона методом микроядерного теста на эритроцитах Danio rerio Медянкина Мария Владимировна Medyankina Mariya Vladimirovna

кандидат биологических наук;

candidate of sciences in biology;

 Кочетков Никита Ильич Kochetkov Nikita Ilyich samatrixs@gmail.com Головачева Наталья Алексеевна Golovacheva Natalya Alexeyevna mirvar@rambler.ru

кандидат ветеринарных наук;

candidate of veterinary sciences;

 Никифоров-Никишин Дмитрий Львович Nikiforov-Nikishin Dmitry L.

кандидат биологических наук;

candidate of sciences in biology;

 МГУТУ им. К.Г. Разумовского Москва Россия MGUTU named after K.G. Razumovsky Moscow Russian Federation Московский государственный университет технологий и управления имени К. Г. Разумовского (Первый казачий университет);Институт общей генетики им. Н. И. Вавилова РАН Россия K. G. Razumovsky Moscow State University of technologies and management (the First Cossack University);Vavilov Institute of General Genetics Russian Academy of Sciences Russian Federation Московский государственный университет технологий и управления имени К. Г. Разумовского (Первый казачий университет) Москва Россия K. G. Razumovsky Moscow State University of technologies and management (the First Cossack University) Moscow Russian Federation Московский государственный университет технологий и управления им. К.Г. Разумовского (ПКУ) Москва Россия Moscow state University University of technology and managing it. Kg Razumovsky (PKU) Moscow Russian Federation ФГБОУ ВО «МГУТУ им. Разумовского (ПКУ)» Москва Россия Moscow State University of Technologies and Management (FCU) Moscow Russian Federation 10 08 2022 10 08 2022 2022 4 71 75 26 05 2022 20 07 2022 https://tsuren.editorum.ru/en/nauka/article/52347/view

В данной работе исследуется генотоксичность (1-(4-chlorophenyl)-3-(2,6-difluoro-benzoyl)urea) методом микроядерного теста на Danio rerio, как стандартном тест-объекте, при концентрациях 0,5; 1 и 2 мг/л. В результате работы было установлено достоверное увеличение частоты встречаемости микроядер (0,73%), при этом другие ядерные аномалии, в максимальных концентрациях эритроцитов, также носили достоверный характер. Было выявлено, что частота микроядер в концентрациях вещества 0,5 и 1 мг/л на пятые сутки опыта была максимальной, при этом в максимальной концентрации (2 мг/л) уровень микроядер был ниже, что, вероятно, связанно с токсическим действием. Повышение уровня микроядер, возможно, связанно с генотоксическим действием продуктов распада DFB. Полученные результаты генотоксичности, полученные с использованием метода микроядерного теста, имели противоречивый характер. По этой причине необходимо проведение дополнительных исследований с использованием метода комет или опытов на клеточных культурах.

In this paper, the genotoxicity of (1-(4-chlorophenyl)-3-(2,6-difluoro-benzoyl)urea) is investigated by a micronuclear test on Danio rerio, as a standard test object, at concentrations of 0.5, 1 and 2 mg/l. As a result of the work, a significant increase in the frequency of occurrence of micronuclei (0.73%) was found, while other nuclear anomalies in the maximum concentrations of erythrocytes were also significant. It was found that the frequency of micronuclei in concentrations of 0.5 and 1 mg/l on the fifth day of the experiment was the maximum, while at the maximum concentration (2 mg/l) the level of micronuclei was lower, which is probably due to toxic effects. An increase in the level of micronuclei may be associated with the genotoxic effect of DFB decay products. The genotoxicity results obtained using the micronucleus test method were contradictory. For this reason, it is necessary to conduct additional studies using the comet method or experiments on cell cultures.

 дифлубензурон (DFB) пестициды генотоксичность микроядерный тест Danio rerio эритроциты генотоксическое действие продуктов распада DFB метод комет опыты на клеточных культурах diflubenzuron (DFB) pesticides genotoxicity micronuclear test Danio rerio erythrocytes genotoxic effect of DFB decay products comet method experiments on cell cultures

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