The method of express determination of the number of microalgae cultures of the genus Tetraselmis
Abstract and keywords
Abstract (English):
The article presents an algorithm for using spectrophotometric measurement to determine the number of cells of cultures of microalgae of the genus Tetraselmis filtered and unfiltered samples. The dependence "density – number of cells" is approximated by a linear equation with a high correlation coefficient, more than 75%, which confirms the high reliability of the method. The ability to measure the density of unfiltered culture samples on a spectrophotometer allows you to quickly estimate the number of cells both in the field and on an industrial scale.

optical density, numbers, microalgae culture, Tetraselmis, mass cultivation
Publication text (PDF): Read Download

1. Kovalev N.N. Cultural and biochemical parameters of microalgae Phaeodactylum tricornutum and Tetraselmis suecica in accumulative cultures / N.N. Kovalev, S.E. Leskova, E.V. Mikheev, Yu.M. Pozdnyakova et al. // Bulletin of the Kamchatka State Technical University. – 2020. – No. 53. – pp. 54-65.

2. Barkia I., Saari N., Manning S.R. Microalgae for high-value products towards human health and nutrition // Marine drugs. – 2019. – Vol. 17(5). – P. 304.

3. Kaparapu J. Application of Microalgae in Aquaculture // Phycological Society. – 2018. – Vol. 48. – Pp. 21-26.

4. Wang G., Xu J., Zeng C. Pelagic microalgae as suitable diets for the benthic harpacticoid copepod Tigriopus japonicas // Hydrobiologia. – 2015. – Vol. 762. – Pp. 81-88.

5. Lubzens E., Zmora O., Stottrup J. et all. Production and nutritional value of rotifers // Live feeds in marine aquaculture. – 2003. – Pp. 300-303.

6. Makridis P., Costa R.A., Dinis M.T. Microbial conditions and antimicrobial activity in cultures of two microalgae species, Tetraselmis chuii and Chlorella minutissima, and effect on bacterial load of enriched Artemia metanauplii // Aquaculture. – 2006. – Vol. 255. – Pp. 76-81.

7. Franklin N.M., Stauber J.L., Lim R.P. Development of flow cytometry-based algal bioassays for assessing toxicity of copper in natural waters // Envir. Toxicol. Chem. – 2001. – Vol. 20. – Pp. 160-170.

8. Günerken E., Hondt E.D., Eppink M. et all. Flow cytometry to estimate the cell disruption yield and biomass release of Chlorella sp. during bead milling // Algal Research. – 2017. – Vol. 25. – Pp. 25-31.

9. Khunjua D.A. Application of spectral-optical methods for characterization of microalgae Scenedesmus quadricauda grown under various conditions / D.A. Khunjua, A.V. Kharcheva, V.A. Terekhova, M.M. Gladkova, etc. // Processes in geomedia. - 2015. – Vol. 1. – Pp. 87-95.

10. Kopytov Yu.P. Method of complex determination of the biochemical composition of microalgae / Yu.P. Kopytov, A.S. Lelekov, R.G. Gevorgiz, M.V. Nekhoroshev et al. // Algologiya. – 2015. – Vol. 25(1). – Pp. 35-40.

11. Sing S.F., Isdepsky A., Borowitzka M.A. et all. Pilot-scale continuous recycling of growth medium for the mass culture of a halotolerant Tetraselmis sp. in raceway ponds under increasing salinity: a novel protocol for commercial microalgal biomass production // Bioresource technology. – 2014. – Vol. 161. – Pp. 47–54.

12. Pereira H., Silva J., Santos T. at all. Nutritional potential and toxicological evaluation of Tetraselmis sp. CTP4 microalgal biomass produced in industrial photobioreactors // Molecules. – 2019. – Vol. 24(17). – P. 3192.

Login or Create
* Forgot password?